Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660867.jpg-250.jpg)
Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram
TES, Sodium Salt, is a high-purity buffering agent available in a 1-kilogram package. This tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid sodium salt is widely used in biochemical and molecular biology research for maintaining stable pH conditions in solutions. TES buffers are particularly effective in biological and clinical applications where precise pH control is essential. The high purity of this reagent makes it suitable for sensitive experiments, including enzyme assays, cell culture, and PCR reactions. TES buffers are used to stabilize proteins, DNA, and other biomolecules, ensuring consistent and reliable results in a variety of laboratory protocols.
FUJIFILM Biosciences RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Apexbio Technology LLC HEPES Sodium salt 75277-39-3 250g
HEPES Sodium salt (CAS 75277-39-3) is a zwitterionic buffering agent designed to stabilize hydrogen ion concentration and maintain physiological pH (6 8 8 2) in biological systems thereby supporting cellular function and biomolecular interactions HEPES Sodium salt exerts its biological activity primarily through non-volatile amphoteric chemical buffering In in vitro and cell-based settings HEPES Sodium salt supports cell viability and experimental consistency by minimizing pH fluctuations Based on these properties HEPES Sodium salt holds research potential in cell-based assays maintenance of cultured mammalian cells controlled in vitro experiments studies of lysosomal biogenesis and investigations of cellular metabolic mechanisms
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 1KG
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 1KG
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 1kg
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culturePurity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 1kg
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-100.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-100.0.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660070.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 100 G
MES Monohydrate (2-(N-Morpholino)ethanesulfonic acid) is a biochemical reagent used as a pH buffer in biological and chemical research. The 100-gram volume is ideal for preparing buffer solutions that maintain stable pH levels in the range of 5.5 to 7.5. MES is widely used in molecular biology, particularly in enzyme assays, protein studies, and cell culture, where precise pH control is critical for maintaining reaction conditions. MES is particularly effective at buffering biological systems, including during electrophoresis and chromatography, where pH stability ensures accurate separation and quantification of proteins and nucleic acids.