Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 500G
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 500G
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659758.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G
HEPES, Free Acid is a critical biochemical reagent for maintaining pH stability in cell cultures and biochemical experiments. This 50g package is ideal for smaller scale applications, where precise pH control is essential for the success of experiments. HEPES is effective in a pH range of 6.8 to 8.2, which makes it a preferred choice for a variety of applications including enzymatic assays, electrophoresis, and cell-based assays. Due to its excellent buffering capacity and low toxicity to cells, HEPES ensures that pH variations are minimized during extended incubation periods, supporting reliable and reproducible experimental outcomes.
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660261.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 1 Kg
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, 1 kg, is a biochemical reagent used to maintain a stable pH in various laboratory applications. It is particularly effective in experiments involving cell culture, enzyme assays, and protein purification. The sodium salt version of MES provides enhanced ionic strength, making it suitable for applications requiring a more robust buffering system. This 1 kg volume is ideal for laboratories with ongoing experiments that require a steady supply of buffer for high-throughput assays or large-scale procedures. MES Sodium Salt ensures consistent pH levels without interfering with the biological systems being studied, making it an essential reagent for molecular biology and biochemistry.
West Pharmaceutical Services Inc WEST PHARMACEUTICAL SERVICES INC
NC3936200 V-35 4432/50G SIL 1000/CS
Chem-Impex International, Inc. Bicine | 150-25-4 | MFCD00004295 | 100G
Bicine, 150-25-4, MFCD00004295, 100G
Bioworld ACES, 250 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5
ALADDIN SCIENTIFIC CORPORATION MES, 100g
useful pH range 5 5 - 6 7 pKa 6 1 MES is one of the Good s buffers the most extensively used biological buffers MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring It does not form complexes with majority of the metals used in environmental and biological studies It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes product description MES is one of the Good s buffers the most extensively used biological buffers MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring It does not form complexes with majority of the metals used in environmental and biological studies It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes
Selleck Chemical LLC Bicine
Bicine (N N-Bis(2-hydroxyethyl)glycine Diethylolglycine Diethanol glycine Dihydroxyethylglycine) is an organic compound used as a buffering agent
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 25g
BICINE | Purity: ≥99% (titration) | MW:163.17 | 150-25-4 | MFCD00004295 | 25g